FractionofbovinebrainGABA-AreceptoraffinitypurifiedagainstimmobilizedbenzodiazepineRo7-1986/1(1988deBlasetalJNeurosciPMID2828565,1988VitoricaetalJNeurosciPMID2828566).TargetinitiallyidentifiedasaGABA-Areceptorby:
-ratbrainimmunocytochemistrystainingpatternvialightmicroscopyidenticaltothatseenvialigandautorADIographywith3H-muscimol
-immunoprecipitationof3H-muscimol-bindingactivityofthepurifiedreceptorcomplex
-recognitionof~57kDaproteininimmunoblotsagainstcruderatbrainmembranefractions
Bindingepitopeidentifiedtobewithinaminoacids26-40(QSVNDPGNMSFVKET,extracellularN-terminus,1992EwertetalBrainRes,PMID1377081)ofhumanGABA-A-R-Beta3(alsoknownasGamma-aminobutyricacidreceptorsubunitbeta-3,accessionnumberP28472)
Mouse:100%identity(15/15aminoacidsidentical)
Rat:100%identity(15/15aminoacidsidentical)
86%identity(13/15aminoacidsidentical,QSVNDPSNMSLVKET)withGABA-A-R-Beta2
66%identity(10/15aminoacidsidentical,HSTNEPSNMSYVKET)withGABA-A-R-Beta1
HybridomakindlydonatedbyAngeldeBlas,UniversityofConnecticut.
Specifications:
Target |  | GABA-A-R-Beta2/3 |
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Applications | | Immunoblot(IB) Immunocytochemistry(ICC) Immuno-goldEM Immunohistochemistry(IHC) Immunoprecipitation(IP) |
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Clone | | 62-3G1 |
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IgGIsotype | | IgG1 |
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SpeciesReactivity | | Bovine(B) Chicken(Chk) Human(H) Mouse(M) Rat(R) |
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Validation | | Br-IB Br-IHC T |
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Type | | TCSupernatant |
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Format | | 5mL |
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CrossReactivity | | Cross-reactswithGABA-A-R-Beta2 Doesnotcross-reactwithGABA-A-RBeta1 |
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ExpectedBandingPattern | | 55kDa |
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CommercialPrice | | 390 |
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Non-ProfitPrice | | 175 |
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DistributorPrice | | PerContract(QUOTE) |
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ABID | | RRID:AB_2315837 |
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